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. 2022 Nov 9;13:6762. doi: 10.1038/s41467-022-34434-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Representative images of immunofluorescence staining and quantification of the relative cardiomyocyte size displayed the block effect of PHLPP2 overexpression on METTL14 knockdown (n = 4 wells/group). Scale bar: 100 μm. b Representative images of immunofluorescence staining and quantification of the Ki67 positive cardiomyocytes displayed the block effect of PHLPP2 overexpression on METTL14 knockdown (n = 4 wells/group). Scale bar: 100 μm. c Representative images of immunofluorescence staining and quantification of the Tunel positive cardiomyocytes displayed the block effect of PHLPP2 overexpression on METTL14 knockdown in the OGD/R-induced apoptosis model (n = 4 wells/group). Scale bar: 100 μm. d Representative western blot and statistical data of NRCMs apoptosis by detection of Bax, Bcl2, Caspase 3, and Cleaved-Caspase 3 in OGD/R induced apoptosis model revealed the block effect of PHLPP2 overexpression on METTL14 knockdown (n = 3 wells/group). e Representative images of immunofluorescence staining and quantification of the Ki67 positive cardiomyocytes revealed the rescue effect of PHLPP2 knockdown on METTL14 overexpression (n = 4 wells/group). Scale bar: 100 μm. f Representative images of immunofluorescence staining and quantification of the Tunel positive cardiomyocytes demonstrated the rescue effect of PHLPP2 knockdown on METTL14 overexpression in the OGD/R-induced apoptosis model (n = 4 wells/group). Scale bar: 100 μm. g Representative western blot and statistical data of NRCMs apoptosis by detection of Bax, Bcl2, caspase 3, and Cleaved-caspase 3 in the OGD/R-induced apoptosis model demonstrated the rescue effect of PHLPP2 knockdown on METTL14 overexpression (n = 3 wells/group). h Representative western blot and statistical data of the phosphorylation levels of Akt-S473 in the OGD/R-induced NRCM apoptosis model displayed the rescue effect of PHLPP2 knockdown on METTL14 overexpression (n = 3 wells/group). Fugw, control without METTL14 overexpression; METTL14 OE, METTL14 overexpression; shScr, Scramble short hairpin RNA; shMETTL14, METTL14 short hairpin RNA to knockdown METTL14; EV, empty vector without PHLPP2 overexpression; PHLPP2, PHLPP2 overexpression; shPHLPP2, PHLPP2 short hairpin RNA to knockdown PHLPP2; OGD/R, oxygen-glucose deprivation/reperfusion; NRCM, neonatal rat cardiomyocyte. All data are expressed as means ± SD. a–h Two-way ANOVA followed by Tukey’s post hoc test. Source data are provided as a Source Data file.