TABLE 4.
Suppression by antiendotoxin peptides of endotoxin-induced TNF-α secretion by RAW 264.7 cells in culture medium containing 10% human serum: effect of the mixing sequence with serum
| Peptide (concn) | TNF-α secretion (pg/ml [mean ± SD]) induced by endotoxina
|
|||
|---|---|---|---|---|
|
E. coli LPS mixing with:
|
P. aeruginosa LPS mixing with:
|
|||
| Peptide first | Serum first | Peptide first | Serum first | |
| LPS control | 4,817 ± 964 | 4,817 ± 964 | 4,428 ± 677 | 4,428 ± 677 |
| LF-33 (2.5 μM) | 575** ± 115 | 1,938* ± 376 | 610** ± 160 | 2,776* ± 478 |
| Polymyxin B (2.5 μM) | 525** ± 130 | 1,259* ± 321 | 883** ± 261 | 4,206 ± 956 |
a
TNF-α secretion induced by endotoxin at 10 ng/ml. *, significantly different from control value at a one-tailed P value of <0.05 in the unpaired t test; **, significantly different from the serum-first value for the same peptide at a one-tailed P value of <0.01 in the unpaired t test.