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. 2022 Nov 9;12:19112. doi: 10.1038/s41598-022-23601-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Infiltration of T cells in HCC samples from transplanted liver patients. (A) Conventional IHC staining of liver-infiltrated T cells in a representative section of paraffin-embedded HCC-bearing liver reveals: (A1) presence of T cell-like CD3⁺ cells (purple dots showing clusters of CD3⁺ cells) in the liver sinusoids and parenchyma, and (A2) FoxP3⁺ cells (brown dots); scale bar 50 µm. (B) Receiver operating characteristic (ROC) curve comparing sensitivity and specificity for the risk index of poor HCC differentiation across a range of FoxP3⁺ infiltrate values.