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. 2022 Nov 9;12:19076. doi: 10.1038/s41598-022-23640-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of 4a on colony formation and cell viability. (a) Representative photos of the colony formation assay after the treatment of A549 cells with increasing concentrations of 4a. (b) Quantification of the colony formation assay. (c) Effect of 4a on cell viability after incubation with compound for 72 h. Data represent the mean ± SEM of n = 3 independent experiments. *p < 0.01, **p < 0.001, ***p < 0.0001, and ****p < 0.00001.