Figure 3.

Restoration of T cell activation of CD28-deficient T cells by miR-17∼92 is cell intrinsic

Adoptive transfer of naive SMARTA⁺ CD4⁺ T cells into CD28^−/− hosts, subsequent LCMV Armstrong infection, and analysis of organs at d8 post-infection. Donor genotypes wt (black), CD28^−/− (purple), rescue (dark blue). Dotted line indicates recipient’s intrinsic Vα2⁺Vβ8.3⁺ population measured in a non-transferred control host.

(A and B) (A) Vα2⁺Vβ8.3⁺ cells from peripheral LN pre-gated on viable, CD3⁺CD4⁺ cells (B) CD44 expression in Vα2⁺Vβ8.3⁺ population from peripheral LN. 2 independent experiments, 4 recipients per group. Error bars represent mean ± SD, Dunn’s multiple comparison test, p values: ns = not significant, ∗<0.05, ∗∗<0.002, ∗∗∗<0.0002. See also Figure S3 for spleen and mesenteric LN.