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. 2022 Oct 20;25(11):105402. doi: 10.1016/j.isci.2022.105402

Figure 3.

Figure 3

Evaluation of expression of key Purkinje markers in control vs PURK-cocktail-treated cells

Immunocytochemistry and fluorescent microscopy showed that the control cells did not express key Purkinje markers [ETV1, IRX3, SCN5a, or PCP4] or the CRISPR-Cas9 Knock-in CNTN2-IRES-mCherry. In contrast, the PURK-cocktail-treated cells strongly expressed ETV1, IRX3, SCN5a, or PCP4 and the CRISPR-Cas9 Knock-in CNTN2-IRES-mCherry reporter gene. DAPI was used to stain the cells' nuclei. The scale bar corresponds to 100 μm in all pictures.