REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ETV1	ThermoFisher	cat# PA589150; RRID:AB_2805388
SCN5a	ThermoFisher	cat# MA1-27429; RRID:AB_1090515
IRX3	ThermoFisher	cat# PA5-111549; RRID:AB_2856959
PCP4	ThermoFisher	cat# 14705-1-AP; RRID:AB_2878075
Alexa 488	ThermoFisher	cat# A32731; RRID:AB_2633280
Alexa 594	ThermoFisher	cat# A-21044; RRID:AB_2535713
Chemicals, peptides, and recombinant proteins
DMEM/F12	SigmaAldrich	cat# D6434-500ML
Glutamax	Gibco	cat# 35050061
FBS	Gibco	cat# 10082147
2-mercaptoethanol	Gibco	cat# 31350010
Anti-anti	Gibco	cat#15240096
mTeSRTM1 basal medium	StemCell Technologies	cat# 85850
hESC-qualified Matrigel	Corning	cat# 354277
Accutase	StemCell Technologies	cat# 07920
Y-27632	StemCell Technologies	cat# 72302
STEMdiffTM Cardiomyocyte Dissociation Kit	StemCell Technologies	cat# 05025
TrueCutTM Cas9 v2	Invitrogen	cat# A36497
Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent	Life Technologies	cat# CMAX00001
Blasticidin	Gibco	cat# A1113902
Rolipram	SigmaAldrich	cat# R6520-10MG
Forskolin	SigmaAldrich	cat# F3917-10MG
CHIR99021	SigmaAldrich	cat# 361571-5MG
SB431542	SigmaAldrich	cat# 616464-5MG
Valproic acid	SigmaAldrich	cat# P4543-10G
RG108	SigmaAldrich	cat# R8279-10MG
Parnate	SigmaAldrich	cat# 616431-500MG
Resveratrol	SigmaAldrich	cat# R5010-100MG
Retinoic Acid	SigmaAldrich	cat# R2625-500MG
Neuregulin	StemCell Technologies	cat# 78071.1
Epinephrine	SigmaAldrich	cat# E4250-5G
NeurobasalTM	Gibco	cat# 12348017
KnockOutTM Serum Replacement	Gibco	cat# 10828010
B-27TM supplement minus vitamin A	Gibco	cat# 12587010
N-2 supplement	Gibco	cat# 17502048
Trypsin-EDTA	Gibco	cat# 25200056
Fibrinogen	SigmaAldrich	cat# F8630
Thrombin	SigmaAldrich	T4648-10KU
D-PBS	Gibco	cat# 14190144
6-aminocaproic acid	Sigma Aldrich	cat# A2504
SILAC DMEM Flex Media, no glucose, no phenol red	Gibco	cat# A2493901
L-Glutamine	Gibco	cat# 25030081
MEM Non Essential Amino Acids	Gibco	cat# 11140050
Sodium DL Lactate	SigmaAldrich	cat# L4263-100ML
STEMdiffTM Cardiomyocyte Support Medium	StemCell Technologies	cat# 05027
Papain from Carica papaya	SigmaAldrich	cat# 76216-50MG
PierceTM 16% Formaldehyde (w/v), methanol free	ThermoScientific	cat# 28908
Triton X-100	Sigma-Aldrich	cat# X100-100ML
10% Normal Goat Serum	ThermoFisher	cat# 50062Z
DAPI solution	ThermoScientific	cat# 62248
DEPC-treated water	Invitrogen	cat# AM9906
PowerTrackTM SYBR Green Master Mix	Applied Biosystems	cat# A46109
Di-4-ANEPPS	Invitrogen	cat#D1199
Critical commercial assays
STEMdiffTM Cardiomyocyte Maintenance Kit	StemCell Technologies	cat# 05020
TrueTagTM DNA Donor System	Life Technologies	cat# A42992
STEMdiffTM Cardiomyocyte Differentiation Kit	StemCell Technologies	cat# 05010
Quick-RNA Microprep kit	Zymo Research	cat# R1050
RNA Clean & Concentrator-5	Zymo Research	cat# R1013
High-Capacity cDNA Reverse Transcriptase kit	Applied Biosystems	cat# 4368814
Deposited data
Raw RNA-seq data Day 4 and Day 7 – PURK cocktail	This paper –Also shared in Figshare	https://doi.org/10.6084/m9.figshare.21290883
Experimental models: Cell lines
AC16-Cardiomyocytes	SigmaAldrich	cat# SCC109
Human iPSCs derived from cardiac fibroblasts	ATCC	cat# ATCC-CYS0105
Oligonucleotides
Homology arms specific to CNTN2	Life Technologies	This paper
Homology arms specific to ACTB	Life Technologies	This paper
Custom TrueGuideTM gRNA for CNTN2	Life Technologies	This paper
Custom TrueGuideTM gRNA for ACTB	Life Technologies	This paper
GAPDH Human qPCR Primer Pair	OriGene	cat# HP205798
CX40 Human qPCR Primer Pair	OriGene	cat# HP208435
CX43 Human qPCR Primer Pair	OriGene	cat# HP200150
ETV1 Human qPCR Primer Pair	OriGene	cat# HP208189
CNTN2 Human qPCR Primer Pair	OriGene	cat# HP208284
HCN2 Human qPCR Primer Pair	OriGene	cat# HP205124
HCN4 Human qPCR Primer Pair	OriGene	cat# HP208604
MEF2C Human qPCR Primer Pair	OriGene	cat# HP206089
ACHE Human qPCR Primer Pair	OriGene	cat# HP231855
TBX3 Human qPCR Primer Pair	OriGene	cat# HP231811
TBX5 Human qPCR Primer Pair	OriGene	cat# HP200177
SCN5a Human qPCR Primer Pair	OriGene	cat# HP234325
IRX3 Human qPCR Primer Pair	OriGene	cat# HP214795
GATA6 Human qPCR Primer Pair	OriGene	cat# HP208427
NKX2-5 Human qPCR Primer Pair	OriGene	cat# HP207706
PCP2 Human qPCR Primer Pair	OriGene	cat# HP218542
PCP4 Human qPCR Primer Pair	OriGene	cat# HP209183
TUBB3 Human qPCR Primer Pair	OriGene	cat# HP209096
OLIG2 Human qPCR Primer Pair	Life Technologies	Watson et al. (2018)
SKOR2 Human qPCR Primer Pair	Life Technologies	Watson et al. (2018)
LHX5 Human qPCR Primer Pair	Life Technologies	Watson et al. (2018)
Recombinant DNA
Custom mCherry-puromycin vector DNA template	Life Technologies	This paper
Software and algorithms
MATLAB	Mathworks	https://www.mathworks.com/products/matlab.html
IncuCyte ZOOM	Sartorius	https://www.essenbioscience.com/en/resources/incucyte-zoom-resources-support/software-modules-incucyte-zoom/
GraphPad Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/
NIS Elements software v4.13	Nikon Instruments	https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research
Fluoview Software	Olympus	https://www.olympus-lifescience.com/en/laser-scanning/fv3000/super-resolution-software-module/
HeatMapper	Babicki et al. (2016)https://doi.org/10.1093/nar/gkw419	http://www.heatmapper.ca/
ElectroMap	O’Shea et al. (2019a)https://doi.org/10.1038/s41598-018-38263-2