Figure 2.

SR-iPSCs generate cells of the three germ layers

(A–C) Representative immunostainings for markers of the three germ layers upon differentiation of SR-iPSCs towards endoderm (A), cardiomyocytes (mesoderm, B), and neural precursors (ectoderm, C).

(D) Negative control: to confirm the specificity of signal, immunostaining of neural precursors was performed excluding primary antibodies. Cells were stained with secondary antibody only. For imaging, the same exposure times as applied in panel C were used. Scale bars: 200 μm (A, C) and 100 μm (B, D).