REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Mouse CD31 (MEC 13.3)	BD Biosciences	Cat# 553370; RRID:AB_394816
Anti-Mouse/Human PAI1 [EPR21850-82]	Abcam	Cat# ab222754
Anti-Mouse/Human Fibronectin antibody	Abcam	Cat# ab2413; RRID:AB_2262874
Anti-Mouse/Human Collagen IV antibody	Abcam	Cat# ab19808; RRID:AB_445160
Anti-Mouse CollagenType VI Antibody	Fitzgerald Industries International	Cat# 70R-CR009X; RRID:AB_1283876
Anti-Mouse CollagenType I	Cedarlane Labs	Cat# CL50151AP-1
Monoclonal Anti-α-Actinin (Sarcomeric) antibody	Sigma-Aldrich	Cat# A7811; RRID:AB_476766
Rabbit IgG Isotype Control (Polyclonal)	R&D	Cat# AB-105-C; RRID:AB_354266
Mouse IgG2a Isotype Control	Biolegend	Cat# 401501
Anti-Rat Alexa fluor 488	Fisher Scientific	Cat# A11006; RRID:AB_2534074
Anti-Rabbit Alexa Fluor 647	Fisher Scientific	Cat# A21245; RRID:AB_2535813
Anti-Rabbit Alexa Fluor 568	Fisher Scientific	Cat# A11011; RRID:AB_143157
Anti-Mouse IgG HRP antibody	Cell Signaling	Cat# 7076P2
Anti-Rabbit HRP antibody	Cell Signaling technology	Cat# 7974S
Chemicals, peptides, and recombinant proteins
Collagenase, Type 2 (Collagenase II)	Worthington-Biochem	Cat# LS004177
Dispase II	Thermo Fisher Scientific	Cat# 17105041
Collagenase D	Sigma-Aldrich	Cat# 11088882001
DMEM/F-12, HEPES medium	Thermo Fisher Scientific	Cat# 11330032
Amphotericin B	Thermo Fisher Scientific	Cat# 15290018
Fetal bovine serum (FBS)	Thermo Fisher Scientific	Cat# 16000044
Bovine Serum Albumin	Sigma Aldrich	Cat# A9418-100G
UltraPure BSA	Thermo Fisher Scientific	Cat# AM2616
Vectashield mounting medium with DAPI	Vector Laboratories	Cat#: H-1200-10
Fast Green FCF	Sigma-Aldrich	Cat#:F7258-25G
Direct Red 80	Sigma-Aldrich	Cat#: 365548-25G
Picric Acid, Saturated	Sigma-Alrdrich	Cat#:5860-16
Xylenes (histological)	Fisher Scientific	Cat#: X3P-1GAL
Ethanol, Absolute (200 Proof)	Fisher Scientific	Cat#: BP2818100
Permount mounting medium	Fisher Scientific	Cat#: SP15-100
Critical commercial assays
Thermo Scientific™ Micro BCA™ Protein Assay Kit	Fisher Scientific	Cat# PI23235
Western Blotting Application Solutions Kit	Cell Signaling Technology	Cat# 12957S
Pierce™ ECL Plus Western Blotting Substrate	Thermo Fisher Scientific	Cat# 32132X3
Deposited data
Raw scRNA-seq data and processed matrices	This paper	GEO: GSE213925
Original western blot images	This paper	Mendeley Data: https://doi.org/10.17632/nptpkvvtjs.1
Experimental models: Organisms/strains
C57Bl/6-NSG	N/A	N/A
mdx-NSG	N/A	N/A
mdxD2-NSG	N/A	N/A
Software and algorithms
Zen 2.6 (blue edition)	Carl Zeiss Microscopy
Prism 9.1.1	GraphPad	https://www.graphpad.com
CellRanger	10X Genomics	Cell Ranger Installation-Software-Single Cell Gene Expression-Official 10x Genomics Support
Seurat Version 3.1.5	Butler et al. (2018)	Release Version 3.1.5 · satijalab/seurat · GitHub
Harmony	Korsunsky et al. (2019)	GitHub - immunogenomics/harmony: Fast, sensitive and accurate integration of single-cell data with Harmony
NicheNet	Browaeys et al. (2020)	GitHub - saeyslab/nichenetr: NicheNet: predict active ligand-target links between interacting cells
Metascape	Zhou et al. (2019)	Metascape
ImageLab versions 5.1 and 6.1	Bio-Rad Labratories