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. 2022 Oct 27;12:1046143. doi: 10.3389/fonc.2022.1046143

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Copyright © 2022 Liu, Tian, Yi, Feng, Chu, Liu, Zhang, Zhang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blotting analysis of PI3K and Akt in PD- and CTX-treated cells. (A) Western blot images. The left column shows protein names, including p-PI3K, PI3K, p-Akt, Akt, and β-actin, which was used as loading control. The top row shows two cell lines HCT116 and LoVo, and drug treatment with different combinations. (B) Bar plots of densitometric analysis. Protein expression levels are normalized to that of the loading control. Columns show the mean ± S.D. One-way ANOVA was used to analyze P values between groups. All groups are compared with the ‘Control’ group, the ‘CTX’ group is compared with the ‘CTX+PD’ group. *P < 0.05 represents a significant difference.