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. 2022 Oct 27;12:1046143. doi: 10.3389/fonc.2022.1046143

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Copyright © 2022 Liu, Tian, Yi, Feng, Chu, Liu, Zhang, Zhang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Combination treatment of CTX and PD decreases proliferation and enhance apoptosis in vivo. (A) Images of Ki67 immunohistochemical analysis and TUNEL staining assay in subcutaneous tumor models. Scale bars, 100 μm. (B) Quantitation of positive immunohistochemical staining for Ki67 and TUNEL staining in every group. Graph of percentage of positive cells for Ki67 and TUNEL staining (three random fields). ImageJ was used to quantify positive immunohistochemical staining results (n=3). Data are shown as the mean ± S.D. One-way ANOVA was used to analyze P values between groups. All groups are compared with the ‘Control’ group, and the ‘CTX’ group is compared with the ‘CTX+PD’ group. *P < 0.05 represents a significant difference.