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. 2022 Oct 20;25(11):105420. doi: 10.1016/j.isci.2022.105420

Figure 4.

Figure 4

Effects of charge conserving mutation of R3 on the open state of the channel

(A and B) Representative rising currents recorded from WT (A) or R3K (B). Currents were measured from a holding potential of −60 mV to test potentials ranging between −60 and +120 mV in 10 mV steps.

(C) The R3K mutation positively shifted the G-V relationship of the channel. Lines indicate fits of the data to a Boltzmann function.

(D) The channel opening time constant Ʈact in WT or R3K mutation. Ʈact was obtained from exponential fit to rising currents. Proton currents were recorded in HEK293 cells expressing Hv1 mutations, pHi = pHo = 6.0. Data are represented as mean ± SEM.