Figure 3.

FA and integrin localization is altered in sacsin KO cells

(A and B) GO term analysis of differentially expressed proteins (A) and phosphorylation (B) in SH-SY5Y cells (p < 0.05, log₂ f.c. cutoff ±0.4).

(C) Confocal images of WT/KO SH-SY5Y cells immunolabeled for vimentin and the FA protein vinculin.

(D) FRAP analysis of the FA protein vinculin in cells expressing tomato-VCL. 2 × 2-μm regions of interest were bleached with a 568-nm laser. Recovery was monitored over 50 cycles of imaging with a 1-s interval. n = 10 cells from each of three independent experiments.

(E) Confocal images of cells immunolabeled for ITGAV. Scale bar, 10 μm.

(F) Scatterplot of statistically significant proteins identified in either SH-SY5Y or primary cortical cultures (p < 0.05, log₂ f.c. cutoff ±0.4). Statistical test: Pearson correlation coefficient.