REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
acetylated tubulin	Cell Signaling	#5335; RRID:AB_10544694
Alexa Fluor 488	Life Technologies	A-11029; RRID:AB_2534088
Alexa Fluor 488	Life Technologies	A-11008; RRID:AB_143165
Alexa Fluor 568	Life Technologies	A-11004; RRID:AB_2534072
Alexa Fluor 568	Life Technologies	A-11011; RRID:AB_143157
Alexa Fluor 633	Life Technologies	A-21052; RRID:AB_2535719
Alexa Fluor 633	Life Technologies	A-21070; RRID:AB_2535731
Alexa Fluor 633 Phalloidin	Life Technologies	A22284
alpha-tub	ThermoFisher	MA1-80017; RRID:AB_2210201
anti-rabbit HRP	Fisher	#PI31466
API	SIGMA	D9542
ATP1A1	Cell Signaling	#3010; RRID:AB_2060983
Beta-Actin	Abcam	ab8226; RRID:AB_306371
BRSK2	Cell Signaling	5460S
CALB1	Novus	NBP2-50028
FAK	CST	71433S; RRID:AB_2799801
Fibronectin	Abcam	ab268020
gamma-tubulin	ThermoFisher	MA1-19421; RRID:AB_1075282
GAPDH	Abcam	ab8245; RRID:AB_2107448
Integrin alpha 6	Abcam	ab235905
Integrin beta1	Abcam	ab134179
Integrin alpha V	Abcam	ab179475; RRID:AB_2716738
IRDye®680 RD	Li-Cor	926–68070
IRDye®680 RD	Li-Cor	926–68071
IRDye®6800 CW	Li-Cor	926–32210
IRDye®6800 CW	Li-Cor	926–32211
ITGA1	BiCell	10001
JNK	CST	9252S; RRID:AB_2250373
KDEL	Enzo Life Sciences	ADI-SPA-827-D
MAP2	Abcam	Ab5392; RRID:AB_2138153
NES	Abcam	Ab22035; RRID:AB_446723
NEUN	Millipore	ABN90P; RRID:AB_2341095
NFH	Abcam	Ab8135; RRID:AB_306298
NUP98	Cell Signaling	#2598; RRID:AB_2267700
p-FAK (Tyr397)	ThermoFisher	44–625G; RRID:AB_2533702
p-JNK (Tyr183/185)	CST	4668S; RRID:AB_823588
p-JUN (Ser 73)	CST	3270S; RRID:AB_2895041
p-Paxillin (Tyr118)	CST	69363S; RRID:AB_2174466
Paxillin	CST	12065S; RRID:AB_2797814
PRKCG	Santa-Cruz	sc-211; RRID:AB_632234
PRPH	Abcam	Ab4666; RRID:AB_449340
PTEN	Santa-Cruz	sc-7974; RRID:AB_628187
Sacsin	Abcam	ab181190
Sacsin	Abcam	Cab80195; RRID:AB_10714400
SYN1	Abcam	Ab8; RRID:AB_2200097
TAU p262	ThermoFisher	44-750-G; RRID:AB_2533743
tau S199	Abcam	Ab81268; RRID:AB_1641106
TAU5	Abcam	Ab80579; RRID:AB_1603723
Vimentin	Abcam	ab8978; RRID:AB_306907
Vimentin	Abcam	ab16700; RRID:AB_443435
Vinculin	ThermoFisher	MA5-11690; RRID:AB_10976821
Chemicals, peptides, and recombinant proteins
Nocodazole	Sigma	#SML1665
all-trans retinoic acid	Sigma	#R2625
Brain-Derived Neurotrophic Factor	Sigma	#B3795
dibutyryl cyclic AMP	Santa Cruz	sc-201567A
MitoTracker Green FM	Invitrogen	#M7514
Critical commercial assays
Tau Aggregation Kit	Cisbio	6FTAUPEG
Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit	Invent	SM-005
Deposited data
PRIDE	This paper	PXD036906
SRA	This paper	GSE214213
Experimental models: Cell lines
SH-SY5Y	this study	ATCC CRL2266
HEK293T	Duncan et al. 2017	ATCC CRL1573
Experimental models: Organisms/strains
C57BL/6-Sacstm2Bebr/J	The Jackson Laboratory	Strain #:033221
Oligonucleotides/primers
WT SACS F: GCTGTCAGGGGGAAATCTGATAAAG	The Jackson Laboratory	N/A
WT SACS R: GCAGCACCTTTAGACAAAAGATTGC	The Jackson Laboratory	N/A
KO SACS F: CAACCTTGGAGAAACTGTGCCTG	The Jackson Laboratory	N/A
KO SACS R: CACCGACGCCAATCACAAACAC	The Jackson Laboratory	N/A
PTEN siRNA Pool	Horizon	5728
Recombinant DNA
hCas9	Addgene	#41815
pGFP-EB1	Addgene	#17234
tdTomato:Vinculin	Addgene	#58146
EGFP-Vimentin-7	Addgene	#56439
Software and algorithms
ImageJ Fiji	NIH	https://imagej.net/software/fiji/downloads
CellProfiler	Broad	www.cellprofiler.org
MaxQuant	Max Planck	https://www.maxquant.org
Zen Black and Zen Blue	Zeiss	https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html