Figure 4.
Estradiol, but not fecal microbiota transplant (FMT) from E2-treated mice supplemented with A. muciniphila, protected against changes in energy intake and expenditure in HFD-fed female mice. (A) Food intake, (B) Water intake, (C) Physical activity, (D) Energy expenditure, (E) VO2 consumption, and (F) VCO2 production were measured in metabolic cages on D18–21 (A–D): *p < 0.05, ANOVA, Tukey post-hoc; (E): *p < 0.05, ANCOVA, Tukey post-hoc). E-E, E2-treated mice receiving FMT from E2 mice (n = 4); V-V, mice with Veh implants receiving FMT from Veh mice (n = 4); V-EA, mice with Veh implants receiving FMT from E2-treated mice supplemented with A. muciniphila (n = 4).