Fig. 4. Naringenin targets cGAS in HSCs.
Mice with CCl4-induced fibrosis were treated with naringenin (100 mg/kg) for 8 weeks (3 times/week). (A) Fluorescence staining of HSC activation indicators α-SMA and cGAS in liver tissue sections. α-SMA and cGAS were coexpressed in liver tissues, and naringenin reduced the expression levels of α-SMA and cGAS in CCl4-induced liver fibrosis. Scale bar 20 µm. (B) Molecular docking of naringenin and cGAS using molecular modeling packages in the Maestro workstation. (A) cGAS, (B) naringenin, (C) Two-dimensional diagram after molecular docking. α-SMA, alpha smooth muscle actin; cGAS, cyclic guanosine monophosphate-adenosine monophosphate synthase.