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. 2022 Apr 28;11(1):26–37. doi: 10.14218/JCTH.2022.00120

Fig. 5. Naringenin inhibits the activation of HSCs in vitro.

Fig. 5

HSC-LX2 were treated with naringenin for 24 h. (A) MTT assays showed that naringenin significant inhibited LX2 at 20 µM and had no effect on L02 cells. (B) Real-time PCR shows that naringenin inhibited expression of theLX2 activation indicators α-SMA and α1-procollagen in a dose-dependent manner. (C) WBs showed that naringenin dose-dependently reduced protein expression of α-SMA and collagen1 in LX2 cells, quantitative analysis, n=3. *p<0.05, versus naringenin (0 µM). (D) Immunofluorescence. Scale bar 20 µm. *p<0.05, versus naringenin (0 µM). α-SMA, alpha smooth muscle actin.