Table 2.
Cell frequencies in healthy children.
| Parent | Population | 1st Qu. | Median | 3rd Qu. | Mean |
|---|---|---|---|---|---|
| Lymphocytes | T cells | 71.65% | 75.67% | 79.11% | 74.99% |
| B cells | 10.93% | 13.42% | 17.12% | 13.82% | |
| Lympho-monocytes | HLADR+LIN- | 10.55% | 13.39% | 16.15% | 14.11% |
| T cells | CD4 T | 54.21% | 59.73% | 65.17% | 59.47% |
| CD8 T | 24.27% | 28.46% | 32.03% | 28.49% | |
| CD4+ CD8+ T | 0.26% | 0.38% | 0.54% | 0.50% | |
| CD4- CD8- T | 8.55% | 10.68% | 14.27% | 11.73% | |
| CD3+ CD56+ T | 1.93% | 3.10% | 5.02% | 4.19% | |
| CD4 T cells | CM CD4 T | 9.43% | 11.83% | 13.69% | 11.85% |
| EM CD4 T | 17.70% | 22.30% | 27.86% | 23.74% | |
| N CD4 T | 52.56% | 59.97% | 67.26% | 58.94% | |
| TEMRA CD4 T | 3.61% | 5.03% | 6.30% | 5.44% | |
| CD127- CD45RA+ CD4 T | 1.01% | 1.73% | 2.49% | 1.95% | |
| CD127+ CD45RA- CD4 T | 25.02% | 30.98% | 39.04% | 32.29% | |
| CD127+ CD45RA+ CD4 T | 57.09% | 63.50% | 69.95% | 62.44% | |
| CD45RA- Foxp3hi activated Treg | 0.61% | 1.33% | 1.74% | 1.33% | |
| CD45RA- FoxP3lo non Treg | 1.43% | 1.86% | 2.57% | 1.97% | |
| CD45RA+ FoxP3lo resting Treg | 1.68% | 2.36% | 2.96% | 2.58% | |
| CD27- CD28- CD4 T | 0.02% | 0.04% | 0.27% | 0.48% | |
| CD27- CD28+ CD4 T | 2.83% | 4.66% | 6.52% | 5.00% | |
| CD27+ CD28+ CD4 T | 93.34% | 95.24% | 96.58% | 94.38% | |
| EM CD4 T cells | EM CD57+ CD4 T | 3.70% | 5.56% | 7.16% | 6.11% |
| EM PD1+ CD4 T | 10.14% | 16.23% | 20.21% | 15.78% | |
| PD1+ CD57+ EM CD4 T | 1.12% | 1.51% | 2.50% | 2.00% | |
| TEMRA CD4 T cellsT cells | TEMRA CD57+ CD4 T | 5.40% | 8.32% | 13.07% | 10.52% |
| CD8 T cells | EM CD8 T | 21.18% | 26.10% | 32.56% | 27.18% |
| N CD8 T | 38.50% | 48.48% | 57.69% | 47.20% | |
| TEMRA CD8 T | 16.05% | 20.03% | 27.97% | 22.47% | |
| CD27- CD28- CD8 T | 1.84% | 4.35% | 10.00% | 7.50% | |
| CD27- CD28+ CD8 T | 0.82% | 1.41% | 2.26% | 2.11% | |
| CD27+ CD28- CD8 T | 9.42% | 14.33% | 19.01% | 15.85% | |
| CD27+ CD28+ CD8 T | 68.04% | 77.64% | 84.29% | 74.52% | |
| EM CD8 T cells | EM CD57+ CD8 T | 7.87% | 12.49% | 18.98% | 14.63% |
| EM PD1+ CD8 T | 6.77% | 12.08% | 18.09% | 14.67% | |
| PD1+ CD57+ EM CD8 TCD8 T | 1.39% | 2.44% | 4.65% | 3.59% | |
| TEMRA CD8 T cells | TEMRA CD57+ CD8 T | 12.88% | 20.37% | 34.62% | 25.18% |
| CD27+ CD28+ CD8 T cells | CD27+ CD28+ CD69+ CD8 T | 2.19% | 3.24% | 6.61% | 5.95% |
| B cells | Double-negative-1 B | 1.31% | 1.66% | 2.20% | 1.92% |
| Double-negative-1 B | Double-negative-2 B | 11.10% | 17.72% | 26.13% | 20.34% |
| IgD+ CD27- B cells | IgM-low naïve B | 60.51% | 66.46% | 70.19% | 64.85% |
| Naïve B | 29.81% | 33.54% | 39.49% | 35.15% | |
| IgM+ IgD+ B cells | Unswitched mem B | 1.91% | 3.35% | 4.97% | 3.63% |
| CD27- IgM+ IgD+ B cellsB cells | Transitional B | 7.47% | 8.00% | 8.71% | 7.92% |
| IgM- IgD+ B cells | IgD only switched mem Bmem B | 12.85% | 18.44% | 32.02% | 23.01% |
| IgM- IgD- B cells | Early plasmablasts | 1.50% | 2.30% | 3.05% | 2.60% |
| Plasmablasts | 3.32% | 4.80% | 7.68% | 5.85% | |
| Switched mem B | 64.78% | 69.39% | 77.40% | 70.13% | |
| CD3- Lymphocytes | CD56dim NK | 30.64% | 41.09% | 49.35% | 39.46% |
| CD56bright NK | 2.33% | 3.37% | 4.40% | 3.59% | |
| CD56dim NK | CD69+ CD56dim NK | 2.51% | 3.48% | 4.93% | 5.06% |
| CD56bright NK | CD69+ CD56bright NK | 24.82% | 28.02% | 31.18% | 29.46% |
| CD3+ CD56+ T cells | CD8+ CD3+ CD56+ T | 47.41% | 55.59% | 71.09% | 58.48% |
| CD14- HLADR+ LIN- | mDCs | 41.07% | 53.63% | 63.95% | 52.21% |
| pDCs | 8.57% | 12.41% | 15.72% | 12.79% | |
| mDCs | CD16 mDCs | 58.35% | 66.47% | 74.12% | 65.13% |
| CD1c CD16 mDCs | 2.87% | 5.12% | 8.02% | 5.57% | |
| CD1c mDCs | 10.46% | 16.66% | 21.49% | 17.44% | |
| HLADR+ LIN- | classical monocytes | 42.50% | 62.02% | 74.95% | 59.16% |
| intermediate monocytes | 3.90% | 8.21% | 19.69% | 12.58% | |
| non-classical monocytes | 6.89% | 10.02% | 14.61% | 10.76% |
Cell frequencies (% of parent) measured by unsupervised clustering within the healthy children cohort (HC, n = 76).