Fig. 3.

Histology of the cisplatin-exposed organoid derived from KS cells.

A: Stereomicroscopic image of cisplatin-exposed organoids derived from KS cells on day 20. The organoids were exposed to 10 µM cisplatin for 144 h. B1: Histology of the tubular structures of the organoids exposed to 10 µM cisplatin for 144 h. HE stained sections; bar=150 µm. B2: Magnified image of B1. Cell death was significant inside the tubular structure (arrows). Cytoplasmic vacuolization was often observed in cells constituting the tubular structure (arrowheads); bar=50 µm. C: Histology of organoids not exposed to cisplatin on day 20. HE-stained; bar=50 µm. D1: Electron microscopy image of organoids derived from KS cells exposed to 10 µM cisplatin for 144 h. Vesicles of low density containing the remains of organelles were often observed (arrows); Bar=5.0 µm. D2: Electron microscopic image of organoids, not exposed to cisplatin at day 20; Bar=5.0 µm. E: Histology of tubular structures of organoids exposed to cisplatin under various conditions. HE-stained; bar=80 µm. Organoids not exposed to cisplatin on days 15 (E1), 16 (E2), and 20 (E3). The organoids were exposed to 20 µM cisplatin for 24 h (E4; from days 14 to 15), 48 h (E5; from days 14 to 16), and 144 h (E6; from days 14 to 20). The organoids were exposed to 30 µM cisplatin for 24 h (E7; from days 14 to 15), 48 h (E8; from days 14 to 16), and 144 h (E9; from days 14 to 20). F: Scoring the cytotoxicity of the organoids under various cisplatin exposure conditions using HE sections. − = No dead cells are present in the tubular structure; + = less than 10% of all the cells in the tubules were dead; ++ = 10 to 50% of all the cells in the tubular structure were dead. +++ = >50% of all the cells in the tubular structure were dead.