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Positions and orientations of the kanamycin cassette (solid lines with arrows) in the region upstream of the galE open reading frame. The positions of the restriction sites are given relative to the galE gene start codon. Open arrows, open reading frames. RKR and NKR, polar insertions of the kanamycin cassette at the EcoRI and NdeI sites, respectively. RKF and NKF, respective nonpolar insertions.
