TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or selective marker | Source or reference |
|---|---|---|
| Strains | ||
| E. coli XL1 BLUE | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tetr)](Con) | Stratagene |
| N. meningitidis NMB | Wild-type clinical isolate; serogroup B; L2, L3, L7, L9 | 14, 22, 25 |
| N. meningitidis NMB ΔgalEt::npt | This study | |
| N. meningitidis MA-1 | Wild-type clinical isolate; serogroup A; L8 | This study |
| N. meningitidis MA-1 ΔgalEt::npt | This study | |
| Plasmids | ||
| pDEADII | Kanamycin | pZERO (Invitrogen) modified by author |
| pBluescript II SK(−) | Ampicillin | Stratagene |
| pBSL14 | Ampicillin and kanamycin | 1 |