Figure 2.

Curcumin and JQ1 singular and coupled treatments impair protein and miR-221-3p and miR-222-3p expression in leukemia cell lines. (A) Reverse transcription-quantitative PCR showed a significant decrease of miR-221-3p in MV-4-11 cells after 48 h of coupled treatment (ANOVA followed by Tukey's post hoc test), while miR-222-3p was not significantly downregulated. (B) Western blot analysis revealing the expression of CDKN1B at protein level after single and coupled treatment with curcumin and JQ1 in protein lysates of AML cell lines. CDKN1B displayed a significant increase in its expression while treated with the combination drugs and mildly with singular drugs. All the western blots were performed after 48 h from treatment with 250 nM of JQ1 and 10 µM curcumin. *P≤0.05, **P≤0.01 and ***P≤0.005.