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. 2022 Oct 26;25(11):105443. doi: 10.1016/j.isci.2022.105443

Figure 3.

Figure 3

HBO1 catalyzes Kbz both in vitro and in mammalian cells

(A) HBO1 catalyzes Kac and Kbz reactions in vitro. Kbz or Kac activities of HBO1 WT/MUT were detected using histones as substrate. Reaction products were detected by western blot with indicated antibodies.

(B) HBO1 catalyzes histone Kbz in mammalian cells. WT and MUT HBO1 were overexpressed alone or in combination with JADE-1 in 293T cells (incubating with 5mM of SB at 24 h after transfection). Whole-cell lysates were collected at 48 h after transfection. Levels of Kbz and Kac were detected by western blot and H3 served as a loading control.

(C) HBO1 catalyzes global Kbz in 293T cells. WT HBO1 was overexpressed in 293T cells and the changes of global Kbz levels were detected by western blot.Data are represented as mean ± SEM, ∗pvalue less than 0.05; ∗∗pvalue less than 0.01; ∗∗∗pvalue less than 0.001.