Figure 2.

Intracellular localization of TMEM16A in myoblast

(A) Localization of transduced GFP-TMEM16A proteins in mouse primary myoblast and myotube (DM4) was determined by confocal microscopy. The myoblasts transfected with GFP were used as a control. Scale bars, 20 μm.

(B) Confocal microscopy images show the co-localization of transduced GFP-TMEM16A and mCherry-Sec61β in the cytosol of mouse primary myoblast, note the surface membrane location of TMEM16A in myotube (DM4). Scale bars, 20 μm.

(C) Co-staining of primary myoblast with TMEM16A and IP₃Rs antibodies. Scale bars, 20 μm. (D) Line scan analysis of part region of myoblast in C panel indicated by white arrow. Black arrows indicate the co-localization of TMEM16A and IP₃Rs signals.