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. 2022 Nov 8;25(11):105446. doi: 10.1016/j.isci.2022.105446

Figure 4.

Figure 4

TMEM16A knockout mice display developmental defects in skeletal muscles

(A) Western blot analysis shows loss of TMEM16A protein expression in the KO skeletal muscle of neonatal mice.

(B) q-RT PCR analysis shows loss of Tmem16a mRNA expression in the KO hind limb muscle of neonatal mice. GAPDH was used as an internal control. n = 3. Data are mean ± SD. ∗∗∗p < 0.001.

(C) Immunofluorescence images show the deletion of TMEM16A protein in limb muscle of neonatal KO mice. Scale bars, 20 μm.

(D) Representative images of P1-, P5-, P10-, and P20-old KO mice and CTR littermates.

(E) Body weight curve of the CTR and KO pups (CTR: n = 15; KO: n = 15). ∗p < 0.05.

(F) 20-day-old CTR and TMEM16A-KO mice were skinned to reveal the lack of muscle in the hind limbs.

(G) Gross morphological analysis of the hind limb and tongue muscles in 5-day-old CTR and KO mice determined by hematoxylin and eosin (H/E) staining of the transverse-sections. Scale bars, 500 μm for hind limb, 100 μm for tongue.

(H) H&E staining shows the nonuniform and unregularly patterned myofibers in neonatal KO mice versus CTR littermates. Scale bars, 20 μm. (I) The skeleton of 10-day-old KO and CTR mice.