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. 2022 Nov 8;25(11):105446. doi: 10.1016/j.isci.2022.105446

Figure 5.

Figure 5

Enhanced proliferation but inhibited myogenic differentiation capacities in TMEM16A deleted myoblasts

(A) EdU staining shows the proliferation capacity of primary myoblasts isolated from the KO and CTR mice. n = 3. Data are mean ± SD. ∗p < 0.05.

(B and C) The increase in expression levels of p-ERK1/2 in the KO versus CTR myoblasts determined by Western blotting. n = 3. Data are mean ± SD. ∗∗∗p < 0.001.

(D and E) The increase in expression levels of Cyclin D in the KO versus CTR myoblasts determined by Western blotting. n = 3. Data are mean ± SD. ∗∗p < 0.01.

(F) Immunostaining with MyHC antibody shows the new formed KO versus CTR myotubes (DM6) differentiated from primary myoblasts. Scale bars, 10 μm.

(G) The fusion index (the percentage of nuclei contained in myosin-positive myotubes) analysis in (F). n = 3. Data are mean ± SD. ∗∗∗p < 0.001.

(H and I) Western blotting shows the expression of MyHC in KO versus CTR myotubes differentiated from the primary myoblasts. n = 3. Data are mean ± SD. ∗p < 0.05.

(J and K) Western blotting shows the expression of Myogenin in KO versus CTR myotubes differentiated from the primary myoblasts. n = 3. Data are mean ± SD. ∗p < 0.05.