REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
TMEM16A	Abcam Rabbit pAb	Cat# ab53212; RRID: AB_883075
L-type Ca ++ CP α1S	Santa Cruz, Mouse mAb	Cat# sc-514685; RRID: AB_2923287
Alpha-Actinin	Boster Biological Technology, Mouse mAb	Cat# BM0003; RRID: AB_2861430
IP3R-I/II/III	Santa Cruz, Mouse mAb	Cat# sc-377518; RRID: AB_2637028
Myosin heavy chain	DSHB, Mouse mAb	Cat# MF20; RRID: AB_2147781
Laminin	Sigma Aldrich, Rabbit pAb	Cat# L9393; RRID: AB_477163
Myogenin	DSHB, Mouse mAb	Cat# F5D; RRID: AB_2146602
RyR	Santa Cruz, Mouse mAb	Cat# sc-376507; RRID: AB_11149759
Cyclin D1	CST, Rabbit pAb	Cat#2978; RRID: AB_2259616
Caspase 3	CST, Rabbit pAb	Cat#9662; RRID: AB_331439
Cleaved Caspase 3	CST, Rabbit pAb	Cat#9661; RRID: AB_2341188
Caspase 12	Santa Cruz, Rat mAb	Cat#sc-21747; RRID: AB_626799
GAPDH	Applied Biological Materials, Mouse mAb	Cat#G041; RRID: AB_2813868
Chemicals, peptides, and recombinant proteins
Bradykinin	MCE	HY-P0206
Caffeine	Calbiochem	58-08-2
LipoMax DNA Transfection Reagent	Sudgen, China	32011
EdU assay kit	Beyotime, China	ST067
PrimeScript RT reagent kit	Takara, Japan	RR037B
HiScript II One Step qRT-PCR SYBR Green Kit	Vazyme, China	Q221-01
Fluo-4 Direct™ Calcium Assay Kit	Thermofisher	F10471
Experimental models: Cell lines
C2C12 myoblasts	ATCC	CVCL_0188
CHO-K1	Institute of Cell Biology, Chinese Academy of Science	https://doi.org/10.1016/j.bbalip.2020.158640
primary myoblasts	This paper	N/A
Experimental models: Organisms/strains
Mice: C57BL/6JGpt	Gem Pharmatech	N000013
Mice: Tmem16a conventional knockout mice	This paper	https://doi.org/10.1016/j.jaci.2017.05.053
Oligonucleotides
Tmem16a –forward: 5′-CTGGCCCCTCTCCTCATTTTCCACAGACAT-3′	Sango Biotech, China	N/A
Tmem16a –reverse: 5′-AGAATTACCCCCTTGGTCTACTATGGCTT-3′	Sango Biotech, China	N/A
Myogenin –forward: 5′-CATCCAGTACATTGAGCGCCTA-3′	Sango Biotech, China	N/A
Myogenin –reverse: 5′-GAGCAAATGATCTCCTGGGTTG-3′	Sango Biotech, China	N/A
GAPDH –forward: 5′-AGGTCGGTGTGAACGGATTTG-3′	Sango Biotech, China	N/A
GAPDH–reverse: 5′-TGTAGACCATGTAGTTGAGGTCA-3′	Sango Biotech, China	N/A
Recombinant DNA
Plasmid: GFP-Tmem16a	Dr. Uhtaek Oh from Seoul National University of Korea	N/A
Plasmid: mCherry-Sec61β	Dr. Haijia Yu from Nanjing Normal University	N/A
Software and algorithms
Adobe Photoshop	https://www.adobe.com	RRID:SCR_014199
ImageJ	https://imagej.net/	RRID:SCR_003070
GraphPad Prism	www.graphpad.com	RRID:SCR_002798