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. 2022 Oct 12;298(11):102588. doi: 10.1016/j.jbc.2022.102588

Figure 4.

Figure 4

CD44v10 facilitates glycolysis by upregulating GLUT1 expression. A, heatmap shows expression profiles of the glycolysis-related genes in BT-549 CD44v10 overexpression cells compared to BT-549 vector cells. Red colors indicate upregulation, and blue indicates downregulation. B, expression validation of candidate genes from heatmap by qPCR. The means ± SD of relative fold changes from triplicate experiments were plotted. β-actin was used as the control. The p values were calculated by paired Student’s t test. C, analysis of GLUT1 expression by Western blot in CD44v10 OE cells. D, knockdown efficiency of GLUT1 was evaluated by Western blotting in CD44v10 OE cells. E, the effects of GLUT1 on glucose consumption and lactate production in BT-549 cells. F, the effects of GLUT1 on ROS levels in BT-549 cells. G, relative levels of phosphorylation of ERK, AKT, and GLUT1 proteins in BT-549 CD44v10 overexpression cells pretreated with ERK (U0126, 20 μM) or AKT inhibitor (LY294002, 20 μM). The ns indicates no significance, ∗∗p <0.01, ∗∗∗p <0.001. GLUT1, glucose transporter 1; ROS, reactive oxygen species.