TABLE 2.
Statistical outcome of the stimulus estrous × genotype × stress ANOVA of all variables measured during the social interaction (SI) test.
| Adults |
Old |
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| Estrous | Estrous × Genotype | Estrous × Stress | Estrous × Genotype × Stress | Estrous | Estrous × Genotype | Estrous × Stress | Estrous × Genotype × Stress | ||||||||||||||||
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| DF | F-value | P-value | DF | F-value | P-value | DF | F-value | P-value | DF | F-value | P-value | DF | F-value | P-value | DF | F-value | P-value | DF | F-value | P-value | DF | F-value | P-value |
| 1–27 | 0.24 | 0.63 | 1–27 | 0.18 | 0.68 | 1–27 | 0.41 | 0.53 | 1–27 | 0.03 | 0.86 | 1–22 | 0.44 | 0.51 | 1–22 | 0.02 | 0.88 | 1–22 | 0.92 | 0.35 | 1–22 | 0.13 | 0.72 |
| 1–30 | 0.003 | 0.96 | 1–30 | 0.23 | 0.63 | 1–30 | 0.002 | 0.97 | 1–30 | 0.05 | 0.82 | 1–25 | 0.01 | 0.92 | 1–25 | 0.07 | 0.79 | 1–25 | 0.03 | 0.87 | 1–25 | 0.39 | 0.54 |
| 1–30 | 0.11 | 0.74 | 1–30 | 0.01 | 0.92 | 1–30 | 0.28 | 0.6 | 1–30 | 0.12 | 0.73 | 1–25 | 0.56 | 0.46 | 1–25 | 0.54 | 0.47 | 1–25 | 0.27 | 0.61 | 1–25 | 0.23 | 0.63 |
| 1–30 | 0.07 | 0.79 | 1–30 | 0.27 | 0.61 | 1–30 | 0.15 | 0.7 | 1–30 | 0.5 | 0.49 | 1–25 | 2.2 | 0.15 | 1–25 | 0.9 | 0.35 | 1–25 | 0.42 | 0.52 | 1–25 | 0.17 | 0.68 |
| 1–30 | 0.06 | 0.81 | 1–30 | 2.45 | 0.13 | 1–30 | 1.22 | 0.28 | 1–30 | 0.32 | 0.57 | 1–25 | 2.29 | 0.14 | 1–25 | 0.15 | 0.7 | 1–25 | 0.09 | 0.76 | 1–25 | 0.27 | 0.61 |
| 1–30 | 0.17 | 0.68 | 1–30 | 0.8 | 0.38 | 1–30 | 1.99 | 1.7 | 1–30 | 1.05 | 0.31 | 1–25 | 0.16 | 0.69 | 1–25 | 0.16 | 0.69 | 1–25 | 0.16 | 0.69 | 1–25 | 0.006 | 0.94 |
| 1–30 | 0.007 | 0.93 | 1–30 | 0.27 | 0.61 | 1–30 | 0.007 | 0.93 | 1–30 | 0.07 | 0.8 | 1–25 | 0.65 | 0.43 | 1–25 | 3.29 | 0.08 | 1–25 | 0.02 | 0.89 | 1–25 | 0.57 | 0.46 |
| 1–30 | 0.003 | 0.95 | 1–30 | 0.09 | 0.77 | 1–30 | 0.02 | 0.89 | 1–30 | 0.7 | 0.41 | 1–25 | 0.43 | 0.52 | 1–25 | 1.81 | 0.19 | 1–25 | 0.73 | 0.4 | 1–25 | 1.04 | 0.32 |
| 1–30 | 0.51 | 0.48 | 1–30 | 0.006 | 0.94 | 1–30 | 0.2 | 0.66 | 1–30 | 1.35 | 0.25 | 1–25 | 0.005 | 0.94 | 1–25 | 1.95 | 0.17 | 1–25 | 2.56 | 0.12 | 1–25 | 0.07 | 0.79 |
| 1–30 | 0.83 | 0.37 | 1–30 | 0.09 | 0.77 | 1–30 | 0.29 | 0.59 | 1–30 | 0.68 | 0.42 | 1–25 | 0.6 | 0.44 | 1–25 | 3.29 | 0.08 | 1–25 | 0.59 | 0.45 | 1–25 | 0.001 | 0.98 |
| 1–30 | <0.001 | 0.99 | 1–30 | 0.12 | 0.73 | 1–30 | 1.84 | 0.18 | 1–30 | 0.01 | 0.91 | 1–25 | 0.08 | 0.78 | 1–25 | 0.41 | 0.52 | 1–25 | 0.74 | 0.4 | 1–25 | 0.85 | 0.37 |
| 1–30 | 0.1 | 0.75 | 1–30 | 1.48 | 0.23 | 1–30 | 0.06 | 0.81 | 1–30 | 1.05 | 0.31 | 1–25 | 0.21 | 0.65 | 1–25 | 1.12 | 0.3 | 1–25 | 1.02 | 0.32 | 1–25 | 0.28 | 0.6 |
NMRI adult stimulus females used during the social interaction (SI) test of the Fmr1 offspring were either in estrous or diestrous phase. The allocation of each stimulus female was balanced across experimental groups so that half of each genotype × stress group encountered either a diestrous or estrous female. Different batches of stimulus female were used for testing at the two ages. No significant effect of estrous or of its interactions was found by the 2 × 2 × 2 (estrous × genotype × stress) ANOVA of all social and USV-related parameters. Slight differences in the exact number of mice (provided in the corresponding figures) are due to technical reasons (e.g., loss of behavioral video recordings) or to the exclusion of outliers (using Grubbs’ ESD test adapted for small sample size). Nb, number; Dur, mean duration; USVs, ultrasonic vocalizations.