. 1999 Mar;67(3):1432–1438. doi: 10.1128/iai.67.3.1432-1438.1999

TABLE 1.

Primer sequences used for PCR-assisted mRNA amplification

Target gene	Oligonucleotide sequence
TNF-α	5′`TTCTGTCTACTGAACTTCGGGGTGATCGGTCC`3′ 5′`GTATGAGATAGCAAATCGGCTGACGGTGTGGG`3′
IFN-γ	5′`TGCATCTTGGCTTTGCAGCTCTTCCTCATGGC`3′ 5′`TGGACCTGTGGGTTGTTGACCTCAAACTTGGC`3′
IL-1α	5′`AAGATGTCCAACTTCACCTTCAAGGAGAGCCG`3′ 5′`AGGTCGGTCTCACTACCTGTGATGAGTTTTGG`3′
IL-4	5′`ATGGGTCTCAACCCCCAGCTAGT`3′ 5′`GCTCTTTAGGCTTTCCAGGAAGTC`3′
IL-10	5′`CTGGAAGACCAAGGTGTCTAC`3′ 5′`GAGCTGCTGCAGGAATGATGA`3′
IL-6	5′`ATGAAGTTCCTCTCTGCAAGAGACT`3′ 5′`CACTAGGTTTGCCGAGTAGATCTC`3′
TGF-β	5′`TGGACCGCAACAACGCCATCTATGAGAAAACC`3′ 5′`TGGAGCTGAAGCAATAGTTGGTATCCAGGGCT`3′
iNOS	5′`CCCTTCCGAAGTTTCTGGCAGCAGC`3′ 5′`GGCTGTCAGAGCCTCGTGGCTTTGG`3′
β-Actin	5′`GTGGGCCGCTCTAGGCACCAA`3′ 5′`CTCTTTGATGTCACGCACGATTTC`3′