Reagent	Stock concentration	Amount
77 μL of NF H2O	N/A	77 μL
10× CutSmart solution	N/A	10 μL
Restriction bridge (5′-CTGCGCCTATACGAATTCGTTATC-3′)	10 μM	3 μL
EcoRI	20 units/μL	10 μL
Total	N/A	100 μL