Target type	Target name	Sequence	Foundations used in replicates
Spot-Tag∗ (peptide target)	Spot-Tag.01	(N-terminus)-PDRVRAVSHWSSGGG-Cys (C-terminus)-3′ATCCCTTCTCTTCCTGTAT ACTAATAGGTGCACGTAGATTC/5Phos/	Fd31, Fd19, Fd20, Fd27, Fd28, Fd29
Bradykinin∗ (peptide target control for non-specific binding)	Brady.01	(N-terminus)-RPPGFSPFR-Cys (C-terminus)-3′ATCCCTTCTCTTCCTGTAT ACTAATAGGTGCACGTAGATTC/5Phos/	Fd12, Fd13, Fd14
DNA∗∗ (null control)	CLR.Null.Block	CTTAGATGCACGTGGATAAT	Fd24, Fd25, Fd26
DNA∗∗ (null control)	5′Phos.01	/5Phos/CTTAGATGCACGTGGATA	Fd7, Fd8, Fd11
DNA∗∗ (positive control)	DNA Target 6.01	/5Phos/CTTAGATGCACGTGGATAATCATA TGTCCTTCTCTTCCCTAATGAAGTAC TAA CCTGA	Fd21, Fd22, Fd23
DNA∗∗ (positive control)	DNA Target 4.01	/5Phos/CTTAGATGCACGTGGATAATCATA TGTCCTTCTCTTCCCTAATAGGATTCC	Fd15, Fd16, Fd17

∗The C-terminal of the peptide targets is directly conjugated to the 3′ end of one DNA tail via a cysteine.

∗∗Binding sequences and DNA tails of DNA targets are continuous oligos rather than conjugated through another chemical conjugation method.