| Reagent | Amount | Stock concentration | Final concentration |
|---|---|---|---|
| Universal Bridge (5′-CTGCGCCTATAGGAATTCGT TATC/i5NitInd//i5NitInd//i5NitInd//i5NitInd// i5NitInd//i5NitInd//i5NitInd//i5NitInd//i5NitInd// i5NitInd//i5NitInd//i5NitInd/GGACACGGC CGTTATC-3′)∗ |
6 μL | 10 μM | 1,200 nM |
| DNA Binder 4.2 (ATACATGGAATCCTAT) | 1 μL | 10 μM | 200 nM |
| DNA Binder 6 (TCAGGTTAGTACTTCAT) | 1 μL | 10 μM | 200 nM |
| DNA Binder 9 (CTTGACTAGTACATGAC CACTTGA) |
1 μL | 10 μM | 200 nM |
| Hybridization Buffer | 41 μL | N/A | N/A |
| Total | 50 μL | N/A | N/A |
∗Each /i5NitInd/ is a 5-Nitroindole, a universal base analogue that exhibits high duplex stability and hybridizes indiscriminately with each of the four natural bases (Loakes and Brown, 1994).