FIGURE 1.

Experimental strategy for the proteomic study of the GP samples from PSP patients, PD patients, and HC individuals. Five batches of 11‐plex TMT experiments were conducted to analyse the proteome of human GP brain tissue samples from 15 PSP, 15 PD, and 15 HC. MP and QC samples were prepared by combining an equal amount of protein from all 45 GP samples. MP was added to each batch after labelling with Tag 11 in one tube. QC was split into five aliquots and processed in each batch separately. TMT tags for individual samples and QC were determined by randomization. The proteins were digested with Lys‐C and trypsin, followed by TMT labelling and prefractionation into 24 fractions prior to mass spectrometry analysis. Proteins were identified by conducting a database search of the acquired mass spectra.