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. 2022 Oct 26;7(44):39760–39771. doi: 10.1021/acsomega.2c03727

Figure 4.

Figure 4

Effects of P2Y2 receptor knockdown on ATP-mediated in vitro invasion and migration. (A, B) CAL27 and SCC15 cells were transfected with three different P2Y2-siRNAs (siRNA1 and siRNA2) and a control siRNA (NC). Western blotting and qRT-PCR were used to evaluate the knockdown efficiency. (C) Colony formation assay was used to evaluate the effect of P2Y2 receptor knockdown on in vitro proliferation after incubation with ATP for 14 days. (D) Transwell invasion assays evaluated the effect of P2Y2 receptor knockdown on in vitro invasion after incubation with ATP for 24 h. The scale bar is 500 μm. (E) Wound healing evaluated the effect of P2Y2 receptor knockdown on in vitro migration after incubation with ATP for 24 h. The scale bar is 1 mm. Data were presented as mean ± SD error bars indicate SD. Three independent experiments were performed (n = 3); t-test was employed for testing the difference between the two groups. ANOVA for testing the distinctions of the groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs control.