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Detection of pathogen nucleic acids with a CRISPR/Cas-based assay to detect L. monocytogenes, M. tuberculosis, and MRSA. Schematic illustration of (a) CAS-EXPAR and (b) CASLFA for the detection of L. monocytogenes, (c) paired dCas9 (PC) reporter system, and (d) CRISPR-MTB for the detection of M. tuberculosis. (e) CRISPR-mediated DNA-FISH and the (f) E-Si-CRISPR technique for the detection of Methicillin-resistant Staphylococcus aureus (MRSA).
