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. 2022 Oct 18;7(44):39562–39573. doi: 10.1021/acsomega.2c04513

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© 2022 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Detection of pathogen nucleic acids with CRISPR/Cas-based assay to detect S. enteritidis, Salmonella, E. coli, P. aeruginosa, and A. baumannii. Schematic representation of (a) allosteric probe-induced catalysis and CRISPR/Cas13a (APC-Cas) system for the detection of S. enteritidis, (b) CRISPR/Cas12a-powered dual-mode biosensor for colorimetric and photothermal-based detection of Salmonella, (c) CRISPR/Cas9-triggered SDA-RCA for the detection of E. coli O157:H7, (d) CIA-based LFB for the detection of P. aeruginosa, and (e) simultaneous detection of multiple genes with Multiplex PCR integrated with the CRISPR/Cas system for A. baumannii.