Figure 2.

Experimental validation of genome-wide screen results

(A) Diagram of arrayed, individual sgRNA validation assay using CRISPRi-iPSCs with exemplar PAX6 flow cytometry histograms for negative and positive screen hits.

(B) Histograms showing PAX6 flow cytometry staining of validated positive and negative hits for both coding and lncRNA classes.

(C) Validation scatterplot of 32 sgRNAs targeting positive and negative hits of both coding and lncRNA classes (16 total targets × 2 replicates × 2 sgRNAs), with x axis showing the genome-wide screen ρ and y axis showing the individual validation ρ′, which were strongly correlated (Pearson r = 0.91).

See also Figure S2.