Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 10;12:19225. doi: 10.1038/s41598-022-23667-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Tight junction proteins are similarly expressed in nAEC and bAEC cells for ALI and biopsy epithelia. (A) Ex vivo cultures of differentiated nAEC and bAEC express similar proportions of the TJ proteins Zonula occludens-1 (ZO-1), Occludin-1 and Claudin-1. (B) A portion of the biopsy sample used to generate the ex vivo models also produced no significant difference for the TJ factors. Blots were sectioned to probe the area corresponding to the molecular weight of the target protein. With the exception of β-actin for the ALI culture Western blot in (A), outcomes were derived from a single transfer membrane for the respective experiments (i.e. ALI vs biopsy) to minimise inconsistencies produced by inter-blot variation. Original blots are in Supplementary Fig. S2 online. Uncertainty intervals are 95% CI; n = 5 per culture group. Values were normalised to the β-actin loading control.