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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Cell Rep. 2022 Sep 27;40(13):111417. doi: 10.1016/j.celrep.2022.111417

Figure 3. FMD cycles improve cognitive behavior in 3xTg mice.

Figure 3.

(A) Experimental diet and behavior schedule for 3xTg males and females starting at 3.5 months of age through 18.5 months of age.

(B) Kaplan-Meier survival curves for 3xTg females in control (n = 25/28), FMD (n = 16/17), and 4% PR (n = 14/16).

(C) Kaplan-Meier survival curves for 3xTg males in control (n = 17/18), FMD (n = 16/17), and 4% PR (n = 14/16) groups.

(D) SAB score (%) for 8.5-month-old C57B/6 WT females (n = 8), 10.5-month-old 3xTg female control (n = 14), FMD (n = 14), and 4% PR (n = 16) groups.

(E) SAB score (%) for 8.5-month-old C57B/6 WT males (n = 8), 10.5-month-old 3xTg male control (n = 13), FMD (n = 16), and 4% PR (n = 15) groups.

(F) Recognition index (RI) scored as a percentage for trial 2 (time in seconds spent exploring novel object versus old object) of NOR task for 8.5-month-old C57B/6 WT females (n = 7), and 10.5-month-old 3xTg female control (n = 14), FMD (n = 13), and 4% PR (n = 13) groups.

(G) RI for trial 2 of NOR task for 8.5-month-old C57B/6 WT males (n = 8), and 10.5-month-old 3xTg male control (n = 14), FMD (n = 14), and 4% PR (n = 16) groups.

Figures 1B and 1C: n = mice that survived to 18 months of age/total mice enrolled in study for group.

Figures 1D–1G: 8.5-month-old C57B/6 WT male and female data shown here are the same data as from Figure 5 and S3 and are shown as a comparison with aging 3xTg mice with or without previous treatment cycles. Data are presented as mean ± SEM. *p < 0.05 and **p < 0.01, one-way ANOVA and Bonferroni post test.