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Cytotoxicity of wt and mutant B. bronchiseptica strains for J774 cells. Bacteria were incubated with J774 cells at an MOI of 10 for 4 h. Cytotoxicity was determined by the release of lactate dehydrogenase as measured with the Cytotox96 kit, and the means and standard errors are presented as percentages of the total lysis by detergent. All strains differ from one another (P < 0.001), except for the wt and ΔcyaA strains and the ΔbscNΔcyaA and Δbvg strains.
