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. 2022 Nov 10;17(11):e0276697. doi: 10.1371/journal.pone.0276697

Table 2. List of genes from RSV and their percent contribution to the RSV derived transcriptome in MRC-5 cells.

Feature Gene order on viral genome 6hpi raw count 6hpi percent of total (percent of NS1) 16hpi raw count 16hpi percent of total (percent of NS1) 24hpi raw count 24hpi percent of total (percent of NS1)
Not identified N/A 563 26.61 8,639 17.26 19,587 19.82
NS1 1 406 19.19 9,078 18.13 18,926 19.16
NS2 2 323 15.26 (80) 5,123 10.23 (56) 10,539 10.67 (56)
N 3 75 3.54 (18) 5,308 10.60 (58) 8,604 8.71 (45)
P 4 201 9.50 (50) 5,537 11.06 (61) 10,111 10.23 (53)
M 5 139 6.57 (34) 4,823 9.63 (53) 8,630 8.73 (45)
SH 6 85 4.02 (21) 2,010 4.01 (22) 4,126 4.18 (22)
G (initiation at 2nd AUG) 7 82 3.88 (20) 1,559 3.11 (17) 3,203 3.24 (17)
F 8 49 2.32 (12) 2,447 4.89 (27) 3,850 3.90 (20)
M2 9 74 3.50 (18) 2,412 4.82 (27) 4,329 4.38 (23)
L 10 0 0 5 0.01 (0) 6 0.01 (0)
Total of all features   2,116 50,064 98,801  

This table illustrates the number of mapped reads that contain the indicated RSV genes as the 5′ most gene. At each timepoint the raw number of individual transcripts is indicated alongside the percent contribution of those transcripts at that timepoint. In addition, we have calculated the percent abundance of each transcript relative to NS1 abundance. Note that this table shows transcripts with a poly A tail over 20nt long.