Table 2. List of genes from RSV and their percent contribution to the RSV derived transcriptome in MRC-5 cells.
Feature | Gene order on viral genome | 6hpi raw count | 6hpi percent of total (percent of NS1) | 16hpi raw count | 16hpi percent of total (percent of NS1) | 24hpi raw count | 24hpi percent of total (percent of NS1) |
---|---|---|---|---|---|---|---|
Not identified | N/A | 563 | 26.61 | 8,639 | 17.26 | 19,587 | 19.82 |
NS1 | 1 | 406 | 19.19 | 9,078 | 18.13 | 18,926 | 19.16 |
NS2 | 2 | 323 | 15.26 (80) | 5,123 | 10.23 (56) | 10,539 | 10.67 (56) |
N | 3 | 75 | 3.54 (18) | 5,308 | 10.60 (58) | 8,604 | 8.71 (45) |
P | 4 | 201 | 9.50 (50) | 5,537 | 11.06 (61) | 10,111 | 10.23 (53) |
M | 5 | 139 | 6.57 (34) | 4,823 | 9.63 (53) | 8,630 | 8.73 (45) |
SH | 6 | 85 | 4.02 (21) | 2,010 | 4.01 (22) | 4,126 | 4.18 (22) |
G (initiation at 2nd AUG) | 7 | 82 | 3.88 (20) | 1,559 | 3.11 (17) | 3,203 | 3.24 (17) |
F | 8 | 49 | 2.32 (12) | 2,447 | 4.89 (27) | 3,850 | 3.90 (20) |
M2 | 9 | 74 | 3.50 (18) | 2,412 | 4.82 (27) | 4,329 | 4.38 (23) |
L | 10 | 0 | 0 | 5 | 0.01 (0) | 6 | 0.01 (0) |
Total of all features | 2,116 | 50,064 | 98,801 |
This table illustrates the number of mapped reads that contain the indicated RSV genes as the 5′ most gene. At each timepoint the raw number of individual transcripts is indicated alongside the percent contribution of those transcripts at that timepoint. In addition, we have calculated the percent abundance of each transcript relative to NS1 abundance. Note that this table shows transcripts with a poly A tail over 20nt long.