Table 5. Contribution to the RSV transcriptome of transcripts with short poly A tails at 24 hpi in MRC-5 cells.
| Feature | Gene order on viral genome | poly A < = 20 raw count | poly A < = 20 percent of total (percent of NS1) | poly A > 20 raw count | poly A > 20 percent of total (percent of NS1) |
|---|---|---|---|---|---|
| Not identified | N/A | 607 | 16.94 | 19,587 | 19.82 |
| NS1 | 1 | 1028 | 28.63 | 18,926 | 19.16 |
| NS2 | 2 | 369 | 10.30 (36) | 10,539 | 10.67 (56) |
| N | 3 | 93 | 2.59 (9) | 8,604 | 8.71 (45) |
| P | 4 | 628 | 17.52 (61) | 10,111 | 10.23 (53) |
| M | 5 | 344 | 9.60 (33) | 8,630 | 8.73 (45) |
| SH | 6 | 36 | 1.00 (4) | 4,126 | 4.18 (22) |
| G (initiation at 2nd AUG) | 7 | 26 | 0.73 (3) | 3,203 | 3.24 (17) |
| F | 8 | 101 | 2.82 (10) | 3,850 | 3.90 (20) |
| M2-1 | 9 | 186 | 5.20 (18) | 4,329 | 4.38 (23) |
| L | 10 | 0 | 0 | 6 | 0.01 (0) |
| Total of all features | 3,584 | 98,801 |
This table illustrates the number of reads mapped that contain the indicated RSV ORFs as the 5′ most ORF. For each ORF raw counts and percent contribution have been calculated for transcripts with a poly A tail over 20nt and those with a poly A tail equal to or less than 20nt.