Table 6. List of genes from RSV and their percent contribution to the RSV derived transcriptome in Calu-3 cells at a single timepoint.
| Feature | Gene order on viral genome | Rep 1 raw count | Rep 1 percent of total (percent of NS1) | Rep 2 raw count | Rep 2 percent of total (percent of NS1) | Rep 3 raw count | Rep 3 percent of total (percent of NS1) |
|---|---|---|---|---|---|---|---|
| Not identified | N/A | 5548 | 6.04 | 5542 | 7.09 | 5031 | 6.47 |
| NS1 | 1 | 21523 | 23.45 | 18363 | 23.48 | 18354 | 23.60 |
| NS2 | 2 | 17765 | 19.35 (83) | 14929 | 19.09 (81) | 14681 | 18.88 (80) |
| N | 3 | 7910 | 8.62 (37) | 6497 | 8.31 (35) | 7076 | 9.10 (39) |
| P | 4 | 8710 | 9.49 (41) | 7052 | 9.02 (38) | 7234 | 9.30 (39) |
| M | 5 | 8470 | 9.23 (39) | 7248 | 9.27 (39) | 6867 | 8.83 (37) |
| SH | 6 | 5809 | 6.33 (27) | 4852 | 6.21 (26) | 4782 | 6.15 (26) |
| G (initiation at 2nd AUG) | 7 | 5530 | 6.02 (26) | 4089 | 5.23 (22) | 5031 | 6.47 (27) |
| F | 8 | 3802 | 4.14 (18) | 2929 | 3.75 (16) | 2997 | 3.85 (16) |
| M2 | 9 | 3191 | 3.48 (15) | 2742 | 3.51 (15) | 2665 | 3.43 (15) |
| L | 10 | 33 | 0.04 (0) | 12 | 0.02 (0) | 29 | 0.04 (0) |
| Total of all features | 91789 | 78194 | 77760 |
This table illustrates the number of reads mapped that contain the indicated RSV genes as the 5′ most gene. For each replicate (rep 1, 2 and 3) the raw number of individual transcripts is indicated alongside their percent contribution of those transcripts in the biological replicate. In addition, we have calculated the percent abundance of each transcript relative to NS1 abundance. Note that this table shows transcripts with a poly A tail over 20nt long