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. 2022 Oct 13;14(20):8221–8242. doi: 10.18632/aging.204337

Figure 7.

Figure 7

AR12 and neratinib cause degradation of p70 S6K in HCN2 neuronal cells. (A) HCN2 cells were transfected with a scrambled siRNA control or with an siRNA to knock down expression of AMPKa. After 24h, cells were treated with vehicle control or with [AR12 (2 μM) + neratinib (50 nM)] for 6h. Cells were fixed in place and immunostaining performed to determine the phosphorylation and expression of the indicated proteins (n = 3 +/-SD). * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control. (B) HCN2 cells were transfected with a scrambled siRNA control or with siRNA molecules to knock down expression of either Beclin1 or ATG5. After 24h, cells were treated with vehicle control or with [AR12 (2 μM) + neratinib (50 nM)] for 6h. Cells were fixed in place and immunostaining performed to determine the phosphorylation and expression of the indicated proteins (n = 3 +/-SD). * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control.