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. 2022 Feb 25;118(14):2960–2972. doi: 10.1093/cvr/cvac023

Figure 1.

Figure 1

Mapping the human foetal heart endothelium using scRNA-seq. (A) Schematic of experimental design for mapping the human foetal heart endothelium using 10× scRNA-seq. (B) Representative FACS gating strategy used to isolate viable CD31+ CD45− ECs. (C) UMAP visualization of clusters identified in scRNA-seq data from cardiac ECs isolated from human foetal heart (n =2). (D) Feature plots showing expression of key marker genes defining distinct endothelial populations. (E) Metagene analysis of foetal heart scRNA-seq data visualized in self-organized maps for total dataset (left) and subpopulations of EC (right). Radar plots show enrichment of each metagene signature in individual clusters. (F) GO term enrichment analysis conducted using genes from metagenes’ signatures A (left) and B (right).