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. 2022 Oct 31;16(10):e0010898. doi: 10.1371/journal.pntd.0010898

Fig 1. Model of congenital Rift Valley fever resulting in vertical transmission in pregnant Sprague Dawley rats.

Fig 1

(A) Sprague Dawley (SD) rats (n = 32) were inoculated subcutaneously (s.c.) with RVFV ZH501 at embryonic day 14 (E14). Eight days later (E22), the rats delivered their pups and placentas were collected for analyses. Dams who survived infection were euthanized at 18- or 22-days post infection (dpi); serum and organs were collected for analyses. Rats meeting euthanasia criteria were immediately euthanized; dam organs, serum, and placentas were collected for analyses. (B) Viral RNA levels by RT-PCR (pfu/mL equivalent) of serum (n = 12 & 11, respectively), uterus (n = 17 and 15, respectively), and ovary (n = 18 and 15, respectively) of infected dams who met euthanasia criteria (died; 2-6dpi) or survived (18-22dpi). (C) Viral RNA levels in placenta samples collected between E20-E22 (n = 44 placentas from n = 17 dams).