Fig 6. Widespread hemorrhaging in the placenta is associated with teratogenic pups.

(A) H&E placentas from uninfected (NI) and infected dams that delivered normal pups or pups with gross signs of teratogenicity. Panels are separated based on placenta regions: decidua, basal zone, and labyrinth zone. Blue and white arrow heads indicate hemorrhaging or necrosis, respectively. 20x images. (B) Pathology scores (scale: 0–4) identifying inflammation, necrosis and hemorrhage severity of H&E stained placentas (E18-E22). (C) Pie graph demonstrating the percentage of placentas displaying inflammation, necrosis, or hemorrhage within the decidua, basal zone, labyrinth zone, or multiple layers. Placentas were grouped into normal or teratogenic based on gross observation. NI, no infection. *** = p < 0.001, **** = p < 0.0001, & n.s. = not significant. ND = none detected. An ANOVA with multiple comparisons was performed to determine statistical significance between groups. NI (n = 18), normal (n = 18), teratogenic (n = 7).