TABLE 1.

Oligonucleotide primers used for PCR

Gene or regiona	Primer sequence (5′→3′)	Restriction site added
lbpB 5′	CATATGAGTACTGTCAAAACCCCCCATA	NdeI
lbpB 3′	AAGCTTATTTATCTTTAACAGCCCCAAAGAC	HindIII
lbpA 5′	CATATGACCACGCACCGCTTAAACCTTGCC	NdeI
lbpA 3′	AAGCTTAAAACTTCATTTCAAGACTGGC	HindIII
orf3 5′	CATATGACCTGTTTACCAAAGACC	NdeI
orf3 3′	AAGCTTAAAACGACCGACCAAGCT	HindIII
lbpB matureb 5′	TCTAGACGCTCTGATGACATCAGCGTC	XbaI
lbpA matureb 5′	AGAAGGGGTTCTGGTGTGGCAGTTTTACCCCTA	XmnI
lbpB internal (717)	TTACATCATGACAAAGGCGATGGC	N/A
lbpA internal (727)	TTTGGTATGAAACGCCACCGAACC	N/A
lbpA internal (738)	TTATATAATGCCACCATCAAAGGGG	N/A
orf3 internal (732)	AGATGACAGCTCATGCGTGGTG	N/A
orf3 internal (728)	AAGGCGTTGATGGGTAAAGGGG	N/A
tbpA 5′ (223)	GCGCAA(TC)GA(AG)GTNACNGGNNTNGGNAAc	N/A
tbpA 3′ (424)	CCCGGGAGATCTTTAAAACTTCATTTCAAGTGC	N/A

^aNumbers in parentheses represent oligonucleotides described in the legend to Fig. 4. 

^bPrimer used for preparing in-frame fusion with the malE gene in the pMalC vector. 

^cN represents an equimolar mixture of the four nucleotides.