TABLE 1.
Oligonucleotide primers and probes used in this study
| Designation | Sequence | Purpose |
|---|---|---|
| US-47 | 5′-GAATGTAACAAAATTATATATTTAAATCTTTGAAAAAT-3′ | Probe for ospE/F loci |
| fla5′ | 5′-GATTATATCAATCATAATACATCAGC-3′ | Specific probe |
| ospC | 5′-CTTATTAAGGTTTTTTTGGACTTTCTGC-3′ | Specific probe |
| ospF | 5′-TACTATAGAAAATAATGGAAAAG-3′ | Specific probe |
| bbk2.11 | 5′-ATCTAAATTAGATGAAAAAG-3′ | Specific probe; PCR |
| 2.10-5′ | 5′-AAAAAAGAAGAGTTGGTTGGTGGTTT-3′ | bbk2.10-specific primer |
| 2.10-3′ | 5′-AAACACCTTGAGCCACTTGCTC-3′ | bbk2.10-specific primer |
| p21 | 5′-GCAAAGTAATAATGAGTTAAAAGTTAAGCAAAG-3′ | Specific probe; PCR |
| ospE | 5′-GGAGACTCTGATACATTCGCAG-3′ | Specific probe; PCR |
| elpA1 | 5′-CAATAGTAGGCGAAATAGCAAG-3′ | Specific probe; PCR |
| elpA2 | 5′-GGAAAGAAATAAAAATGTTGTAGGG-3′ | Specific probe; PCR |
| ospA | 5′-TATGTTCTTGAAGGAACTCTAACTGCTGAAAAAACAACATT-3′ | Specific probe |
| Cons-DS | 5′-CCCTTTACTATTTTTGTAGTTGCTTTTGC-3′ | Downstream PCR primer |
| 2.9-5′consL | 5′-AAAGGAGTAACAATGAAAATYATCAAC-3′a | Long-distance PCR |
| ospE-R+C | 5′-CCTGCGAATGTATCAGAGTCTCC-3′ | Long-distance PCR |
| p21-R+C | 5′-CCCGGAATCCTTAATTTTATTTCC-3′ | Long-distance PCR |
| ospF-LD | 5′-CATCTATAACTTTGTTTGCCAATTCATTGC-3′ | Long-distance PCR |
a
Y represents a C or T nucleotide.